To analyze a reasonable cut-off value of serum AFP in hepatic cancer diagnosis, 35 cases of hepatic cancer patients and 35 healthy people were chosen as the samples. Electrochemiluminescence immunoassay (ECLIA) and enzyme-1inked immunosorbent assay (ELISA) are used to collect serum samples and analyze AFP levels. SPSS 18.0 software was used for statistical analysis. The correlation coefficient is 0.978, showing the results by ECLIA were significantly correlated to those by ELISA. They had no significant difference, P＞0.05. The area under the ROC curve was 0.890, 0.880 respectively by these two methods. The sensitivity, specificity and accuracy were 74.3%, 94.3%, 84.3% by ECLIA if taking 3.25 μg/L as cut-off value and 62.9%, 100%, 81.4% by ELISA if taking 1.118 μg/L as cut-off value. Whereas if taking 20 μg/L as cut-off value, the sensitivity and accuracy were only 45.7%, 72.9% by ECLIA and 48.6%, 74.3% by ELISA. So decreasing cut-off value of serum AFP can improve the diagnosis value of hepatic cancer effectively.